AFM Workshop


The morphological effect of a fraction of the extract of T. fagifolia on S. aureus ATCC 29213 cells is shown in the figure below. Changes in the appearance in the cell envelope of treated bacteria are notable: it was found that the treated bacteria had increased roughness on the cell surface compared to the smooth surfaces of the nontreated bacteria, and treated bacteria significantly increased in size.

Atomic Force Microscopy Study of Staphylococcus Aureus treatedAFM height images showing S. aureus ATCC 29213 before (A & B) and after (C&D) exposure to the extract from T. fagigolia.

The work was carried out by doctoral student Alyne Araujo, under the supervision of Dr. José Roberto Leite, of the Biotec (Centre for Biodiversity Research and Biotechnology) research group in the city of Parnaíba, Brazil. Said Dr. Leite:

"The images showed for the first time alterations caused by these natural products in bacteria of this genus. The TT AFM has been in our lab a short time, and is already producing published results in a number of projects."

The project of Biotec is in partnership with researchers from the UCIBIO@Requimte lab, University of Porto, Portugal. The publication of this work is available online.

Alyne Araujo & TT AFMDoctoral student Alyne Araujo with the TT AFM in Brazil's Centre for Biodiversity Research and Biotechnology, Parnaíba

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